Multi-transgenic pigs for xenotransplantation
نویسندگان
چکیده
منابع مشابه
Progress in Xenotransplantation Research Employing Transgenic Pigs
Microinjection of foreign DNA into pronuclei of a fertilized oocyte has predominantly been used for the generation of transgenic livestock. This technology works reliably, but is inefficient and results in random integration and variable expression patterns in the transgenic offspring. Nevertheless, remarkable achievements have been made with this technology with regard to xenotransplantation. ...
متن کاملFunction Measurements of HLA-II Transgenic Pigs for Xenotransplantation
Hao-Chih Tai1, Ching-Fu Tu2,*, Tien-Shuh Yang2, Jang-Ming Lee1, San-Yuan Huang2,3 and Bao-Tyan Wang4 1Department of Surgery, National Taiwan University Hospital and College of Medicine 2Divisions of Biotechnology and Applied Biology, Animal Technology Institute Taiwan 3Department of Animal Science, National Chung Hsing University, Taichung 4Department of Genomic Medicine, Changhua Christian Hos...
متن کاملDistribution and expression of porcine endogenous retroviruses in multi-transgenic pigs generated for xenotransplantation.
BACKGROUND Multi-transgenic pigs produced for use in xenotransplantation have to be screened for the presence and expression of porcine endogenous retroviruses (PERV) to select animals with low PERV load. The production of transgenic pigs may also be associated with the integration of the transgene adjacent to or into the locus of a PERV provirus, potentially leading to an enhanced virus expres...
متن کاملModifying the Glycome in Pigs for Xenotransplantation.
I of glycosyltransferase genes during human evolution is a fundamental cause of differential glycosylation in humans and pigs, resulting in the presence of carbohydrate structures on pig cells that are recognized by natural (preexisting) human antibodies. αGal, the first of these epitopes to be identified, is synthesized by α1,3galactosyltransferase, encoded by theGGTA1 gene. The crucial import...
متن کاملEfficient production of multi-modified pigs for xenotransplantation by ‘combineering’, gene stacking and gene editing
Xenotransplantation from pigs could alleviate the shortage of human tissues and organs for transplantation. Means have been identified to overcome hyperacute rejection and acute vascular rejection mechanisms mounted by the recipient. The challenge is to combine multiple genetic modifications to enable normal animal breeding and meet the demand for transplants. We used two methods to colocate xe...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Xenotransplantation
سال: 2013
ISSN: 0908-665X
DOI: 10.1111/xen.12014_19